制备方法

用节链霉菌菌株为菌种，在含有碳水化合物和有机氮源的液体培养基中进行通气深层发酵，当达到相当效价单位后，由发酵液中提取两性霉素。两性霉素含有A、B两种成分，A成分毒性小，抗真菌作用微弱，不用于临床，B成分的作用强，称两性霉素B。

合成制备方法

用节链霉菌菌株为菌种，在含有碳水化合物和有机氮源的液体培养基中进行通气深层发酵，当达到相当效价单位后，由发酵液中提取两性霉素。两性霉素含有A、B两种成分，A成分毒性小，抗真菌作用微弱，不用于临床，B成分的作用强，称两性霉素B。

用途简介

细胞培养用防霉剂（抑制霉菌和酵母菌生长），推荐浓度 5μg/ml培养液。细胞中毒浓度为30μg/ml培养液。

用途

细胞培养用防霉剂（抑制霉菌和酵母菌生长），推荐浓度2.5μg/ml培养液。细胞中毒浓度为30μg/ml培养液。                         

包装等级：

III

风险类别：

6.1(b)

WGK_Germany：

3
德国有关水污染物质的分类清单

危险类别码：

R36/37/38

安全说明：

S26-S36/37/39-S45-S36

RTECS号：

BU2625000

危险标志：

Xi:Irritant

CHEMICAL IDENTIFICATION

RTECS NUMBER :

BU2625000

CHEMICAL NAME :

Amphotericin B

CAS REGISTRY NUMBER :

1397-89-3

BEILSTEIN REFERENCE NO. :

0078342

LAST UPDATED :

199612

DATA ITEMS CITED :

33

MOLECULAR FORMULA :

C47-H73-N-O17

MOLECULAR WEIGHT :

924.21

WISWESSER LINE NOTATION :

T6-36- A AO RVO A&U C&U E&U G&U I&U K&U M&UTJ CVQ DQ FQ HQ JQ KQ NQ PQ T1 U1 VQ W1 O&O- BT6OT

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :

LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Human - woman

DOSE/DURATION :

22 mg/kg/4D-I

TOXIC EFFECTS :

Blood - leukopenia

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Human - woman

DOSE/DURATION :

20 ug/kg

TOXIC EFFECTS :

Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :

LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Human - man

DOSE/DURATION :

164 ug/kg/5H-I

TOXIC EFFECTS :

Cardiac - other changes

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Human - infant

DOSE/DURATION :

15 mg/kg/4D-I

TOXIC EFFECTS :

Nutritional and Gross Metabolic - changes in potassium Biochemical - Enzyme inhibition, induction, or change in blood or tissue levels - transaminases

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Human - woman

DOSE/DURATION :

1 mg/kg/1H-C

TOXIC EFFECTS :

Cardiac - pulse rate

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Oral

SPECIES OBSERVED :

Rodent - rat

DOSE/DURATION :

>5 gm/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intraperitoneal

SPECIES OBSERVED :

Rodent - rat

DOSE/DURATION :

>5 gm/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Rodent - rat

DOSE/DURATION :

1600 ug/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intramuscular

SPECIES OBSERVED :

Rodent - rat

DOSE/DURATION :

>5 gm/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Oral

SPECIES OBSERVED :

Rodent - mouse

DOSE/DURATION :

>8 gm/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intraperitoneal

SPECIES OBSERVED :

Rodent - mouse

DOSE/DURATION :

27740 ug/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Rodent - mouse

DOSE/DURATION :

1200 ug/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intramuscular

SPECIES OBSERVED :

Rodent - mouse

DOSE/DURATION :

>5 gm/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :

LD - Lethal dose

ROUTE OF EXPOSURE :

Unreported

SPECIES OBSERVED :

Rodent - mouse

DOSE/DURATION :

>50 mg/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Mammal - dog

DOSE/DURATION :

6 mg/kg

TOXIC EFFECTS :

Kidney, Ureter, Bladder - other changes

TYPE OF TEST :

LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Rodent - rabbit

DOSE/DURATION :

5 mg/kg

TOXIC EFFECTS :

Behavioral - tremor Behavioral - convulsions or effect on seizure threshold

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Intraperitoneal

SPECIES OBSERVED :

Rodent - rat

DOSE/DURATION :

490 mg/kg/14W-I

TOXIC EFFECTS :

Blood - changes in serum composition (e.g. TP, bilirubin, cholesterol) Blood - changes in erythrocyte (RBC) count Nutritional and Gross Metabolic - weight loss or decreased weight gain

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Mammal - dog

DOSE/DURATION :

55 mg/kg/13W-I

TOXIC EFFECTS :

Kidney, Ureter, Bladder - urine volume increased Kidney, Ureter, Bladder - changes in bladder weight Blood - changes in erythrocyte (RBC) count

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Mammal - dog

DOSE/DURATION :

37 mg/kg/30D-I

TOXIC EFFECTS :

Kidney, Ureter, Bladder - changes in tubules (including acute renal failure, acute tubular necrosis) Nutritional and Gross Metabolic - weight loss or decreased weight gain

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Intravenous

DOSE :

5500 ug/kg

SEX/DURATION :

female 6-16 day(s) after conception

TOXIC EFFECTS :

Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Oral

DOSE :

600 mg/kg

SEX/DURATION :

male 30 day(s) pre-mating

TOXIC EFFECTS :

Reproductive - Paternal Effects - prostate, seminal vesicle, Cowper's gland, accessory glands

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Intravenous

DOSE :

20 mg/kg

SEX/DURATION :

male 10 day(s) pre-mating

TOXIC EFFECTS :

Reproductive - Paternal Effects - spermatogenesis (incl. genetic material, sperm morphology, motility, and count)

MUTATION DATA

TEST SYSTEM :

Rodent - rabbit

DOSE/DURATION :

20 mg/kg/11D

REFERENCE :

JRPFA4 Journal of Reproduction and Fertility. (Biochemical Soc. Book Depot, POB 32, Commerce Way, Colchester, Essex CO2 8HP, UK) V.1- 1960- Volume(issue)/page/year: 7,13,1964 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 84491 No. of Facilities: 561 (estimated) No. of Industries: 2 No. of Occupations: 2 No. of Employees: 6392 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 84491 No. of Facilities: 386 (estimated) No. of Industries: 2 No. of Occupations: 10 No. of Employees: 15048 (estimated) No. of Female Employees: 12218 (estimated)

生态数据：

对是水稍微有危害的不要让未稀释或大量的产品接触地下水、水道或者污水系统，若无政府许可，勿将材料排入周围环境。

分子结构数据

1、   摩尔折射率：240.24

2、   摩尔体积（m³/mol）：689.4

3、   等张比容（90.2K）：2006.1

4、   表面张力（dyne/cm）: 71.6

5、   介电常数：无可用

6、   偶极距（10^-24cm³）：无可用

7、   极化率：95.24

计算化学数据

1.疏水参数计算参考值（XlogP）:0

2.氢键供体数量:12

3.氢键受体数量:18

4.可旋转化学键数量:3

5.互变异构体数量:2

6.拓扑分子极性表面积320

7.重原子数量:65

8.表面电荷:0

9.复杂度:1670

10.同位素原子数量:0

11.确定原子立构中心数量:19

12.不确定原子立构中心数量:0

13.确定化学键立构中心数量:7

14.不确定化学键立构中心数量:0

15.共价键单元数量:1