Section I.Chemical Product and Company Identification
Chemical Name Crystal Violet
[Ion association reagent for spectrophotometric analysis.]
Portland OR
Basic Violet 3
Synonym
Chemical Formula C25H30ClN3•9H2O
CAS Number 548-62-9
Section II. Composition and Information on Ingredients
Chemical Name CAS Number Percent (%) TLV/PEL Toxicology Data
Crystal Violet 548-62-9 ---------- This chemical is classified as a Rat LD50 (oral) 420mg/kg
[Ion association reagent for spectrophotometric analysis.]
carcinogen. There is no Mouse LD50 (oral) 96mg/kg
acceptable exposure limit for a Rabbit LD50 (intraperitoneal)
carcinogen. 5mg/kg
Section III. Hazards Identification
Acute Health Effects Toxic if ingested or inhaled. Avoid prolonged contact with this material. Overexposure may result in serious illness or death.
Irritating to eyes and skin on contact. Inhalation causes irritation of the lungs and respiratory system. Inflammation of the
eye is characterized by redness, watering, and itching. Skin inflammation is characterized by itching, scaling, reddening, or,
occasionally, blistering.
Follow safe industrial hygiene practices and always wear proper protective equipment when handling this compound.
CARCINOGENIC EFFECTS :
Chronic Health Effects
Rat-TDLo - ROUTE: Oral; DOSE: 10950 mg/kg/2Y continuous
TOXIC EFFECTS:
Tumorigenic - Carcinogenic by RTECS criteria
Endocrine - Thyroid tumors
Mouse-TDLo - ROUTE: Oral; DOSE: 25750 mg/kg/2Ycontinuous
TOXIC EFFECTS:
Tumorigenic - Carcinogenic by RTECS criteria
Kidney, Ureter, and Bladder - Tumors
Tumorigenic Effects - Ovarian tumors
MUTAGENIC EFFECTS : Not available.
TERATOGENIC EFFECTS :
DEVELOPMENTAL TOXICITY:
Rat-TDLo - ROUTE: Oral; DOSE: 100 mg/kg; DURATION: female 6-15D of pregnancy
TOXIC EFFECTS:
Specific Developmental Abnormalities -Musculoskeletal system
Specific Developmental Abnormalities -Urogenital system
Rat-TDLo - ROUTE: Oral; DOSE: 100 mg/kg; DURATION: female 6-15D of pregnancy
TOXIC EFFECTS:
Effects on Embryo or Fetus - Fetotoxicity (except death, e.g., stunted fetus)
Effects on Embryo or Fetus - Fetal death
Specific Developmental Abnormalities -
Musculoskeletal system
Repeated exposure to an highly toxic material may produce general deterioration of health by an accumulation in one or
many human organs.
Section IV. First Aid Measures
Eye Contact Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15
minutes. Get medical attention.
Skin Contact In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing
and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention immediately.
Inhalation If the victim is not breathing, perform mouth-to-mouth resuscitation. Loosen tight clothing such as a collar, tie, belt or
waistband. If breathing is difficult, oxygen can be administered. Seek medical attention if respiration problems do not
improve.
Continued on Next Page
Crystal Violet
[Ion association reagent for spectrophotometric analysis.]
Ingestion INDUCE VOMITING by sticking finger in throat. Lower the head so that the vomit will not reenter the mouth and throat.
Loosen tight clothing such as a collar, tie, belt or waistband. If the victim is not breathing, perform mouth-to-mouth
resuscitation. Examine the lips and mouth to ascertain whether the tissues are damaged, a possible indication that the toxic
material was ingested; the absence of such signs, however, is not conclusive.
Section V. Fire and Explosion Data
Not available.
May be combustible at high temperature. Auto-Ignition
Flammability
Flammable Limits Not available.
Flash Points Not available.
Combustion Products These products are toxic carbon oxides (CO, CO2), nitrogen oxides (NO, NO2), halogenated compounds.
WARNING: Highly toxic HCl gas is produced during combustion.
Fire Hazards Not available.
Risks of explosion of the product in presence of mechanical impact: Not available.
Explosion Hazards
Risks of explosion of the product in presence of static discharge: Not available.
Fire Fighting Media SMALL FIRE: Use DRY chemical powder.
and Instructions LARGE FIRE: Use water spray, fog or foam. DO NOT use water jet.
Consult with local fire authorities before attempting large scale fire-fighting operations.
Section VI. Accidental Release Measures
Spill Cleanup Environmentally hazardous material. Toxic material. Irritating material. Carcinogenic material. Light-sensitive material.
Stop leak if without risk. DO NOT get water inside container. DO NOT touch spilled material. Use water spray to reduce
Instructions
vapors. Prevent entry into sewers, basements or confined areas; dike if needed. Eliminate all sources of ignition. Consult
federal, state, and/or local authorities for assistance on disposal.
Section VII. Handling and Storage
ENVIRONMENTAL HAZARD. TOXIC. IRRITANT. CARCINOGEN. LIGHT-SENSITIVE. Keep locked up. Keep away from
Handling and Storage
heat. Mechanical exhaust required. When not in use, tightly seal the container and store in a dry, cool place. Avoid
Information
excessive heat and light. DO NOT ingest. Do not breathe dust. Wear suitable protective clothing. If ingested, seek medical
advice immediately and show the container or the label. Treat symptomatically and supportively.
Always store away from incompatible compounds such as oxidizing agents.
Section VIII. Exposure Controls/Personal Protection
Engineering Controls Use process enclosures, local exhaust ventilation, or other engineering controls to keep airborne levels below recommended
exposure limits. If user operations generate dust, fume or mist, use ventilation to keep exposure to airborne contaminants
below the exposure limit.
Personal Protection Splash goggles. Lab coat. Dust respirator. Gloves. A MSHA/NIOSH approved respirator must be used to avoid inhalation of
the product. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this product.
This chemical is classified as a carcinogen. There is no acceptable exposure limit for a carcinogen.
Exposure Limits
Section IX. Physical and Chemical Properties
Physical state @ 20°C Green crystal. Solubility Not available.
Not available.
Specific Gravity
Molecular Weight 407.98 (Anh) Partition Coefficient Not available.
Boiling Point Not available. Not available.
Vapor Pressure
Melting Point Not available. Vapor Density Not available.
Not available. Not available.
Refractive Index Volatility
Critical Temperature Not available. Odor Not available.
Not available. Not available.
Viscosity Taste
Section X. Stability and Reactivity Data
This material is stable if stored under proper conditions. (See Section VII for instructions)
Stability
Conditions of Instability Avoid excessive heat and light.
Incompatibilities Reactive with strong oxidizing agents, strong reducing agents.
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Crystal Violet
[Ion association reagent for spectrophotometric analysis.]
Section XI. Toxicological Information
BO9000000
RTECS Number
Eye contact. Ingestion. Inhalation. Skin contact.
Routes of Exposure
Toxicity Data Rat LD50 (oral) 420mg/kg
Mouse LD50 (oral) 96mg/kg
Rabbit LD50 (intraperitoneal) 5mg/kg
Chronic Toxic Effects CARCINOGENIC EFFECTS :
Rat-TDLo - ROUTE: Oral; DOSE: 10950 mg/kg/2Y continuous
TOXIC EFFECTS:
Tumorigenic - Carcinogenic by RTECS criteria
Endocrine - Thyroid tumors
Mouse-TDLo - ROUTE: Oral; DOSE: 25750 mg/kg/2Ycontinuous
TOXIC EFFECTS:
Tumorigenic - Carcinogenic by RTECS criteria
Kidney, Ureter, and Bladder - Tumors
Tumorigenic Effects - Ovarian tumors
MUTAGENIC EFFECTS : Not available.
TERATOGENIC EFFECTS :
DEVELOPMENTAL TOXICITY:
Rat-TDLo - ROUTE: Oral; DOSE: 100 mg/kg; DURATION: female 6-15D of pregnancy
TOXIC EFFECTS:
Specific Developmental Abnormalities -Musculoskeletal system
Specific Developmental Abnormalities -Urogenital system
Rat-TDLo - ROUTE: Oral; DOSE: 100 mg/kg; DURATION: female 6-15D of pregnancy
TOXIC EFFECTS:
Effects on Embryo or Fetus - Fetotoxicity (except death, e.g., stunted fetus)
Effects on Embryo or Fetus - Fetal death
Specific Developmental Abnormalities -
Musculoskeletal system
Repeated exposure to an highly toxic material may produce general deterioration of health by an accumulation in one or many
human organs.
Acute Toxic Effects Toxic if ingested or inhaled. Avoid prolonged contact with this material. Overexposure may result in serious illness or death.
Irritating to eyes and skin on contact. Inhalation causes irritation of the lungs and respiratory system. Inflammation of the eye
is characterized by redness, watering, and itching. Skin inflammation is characterized by itching, scaling, reddening, or,
occasionally, blistering.
Follow safe industrial hygiene practices and always wear proper protective equipment when handling this compound.
Section XII. Ecological Information
Ecotoxicity Not available.
Hexamethyl-p-rosaniline chloride's production and uses as a dye and topical antibacterial medication, both human and
Environmental Fate
veterinary, may result in its release to the environment through various waste streams. If released to air, an estimated vapor
pressure of 1.9X10-14 mm Hg at 25 deg C indicates hexamethyl-p-rosaniline chloride will exist solely in the particulate phase
in the ambient atmosphere. Particulate-phase hexamethyl-p-rosaniline chloride may be removed from the atmosphere by wet
and dry deposition. Hexamethyl-p-rosaniline chloride has a UV max of 590 nm indicating a potential for direct photolysis in the
environment. If released to soil, hexamethyl-p-rosaniline chloride is expected to have no mobility based upon an estimated
Koc of 4X10+6. Hexamethyl-p-rosaniline chloride is a cationic salt and cations generally adsorb to organic carbon and clay
more strongly than their neutral counterparts. Volatilization from moist soil surfaces is not expected to be an important fate
process based upon an estimated Henry's Law constant of 3.1X10-16 atm-cu m/mole. If released into water,
hexamethyl-p-rosaniline chloride isexpected to adsorb to suspended solids and sediment based upon the estimated Koc.
Measurable transformation rates of 0.002 to 0.012/hour, that correspond to a half-life range of 2.4 to 14.4 days, were
observed in 42 batch culture die-away tests using natural water samples from four sites. The photoreaction of
hexamethyl-p-rosaniline chloride in water is reported to give p-dimethylamino phenol, 4,4'-bis dimethylamino benzophenone,
the leuco- and demethylated derivative of hexamethyl-p-rosaniline chloride. Volatilization from water surfaces is not expected
to be an important fate process based upon this compound's estimated Henry's Law constant. An estimated BCF of 3.2
suggests the potential for bioconcentration in aquatic organisms is low. Occupational exposure to hexamethyl-p-rosaniline
chloride may occur through inhalation of dust and dermal contact with this compound at workplaces where
hexamethyl-p-rosaniline chloride is produced or used. General population exposure may occur via use of topical medications
and consumer products containing this compound. (SRC) [Peer Reviewed]
Section XIII. Disposal Considerations
Recycle to process, if possible. Consult your local or regional authorities. You may be able to dissolve or mix material with a
Waste Disposal
combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber system. Observe all
federal, state, and local regulations when disposing of the substance.
Section XIV. Transport Information
DOT Classification CLASS 9: Miscellaneous hazardous material.
UN: 3077
PIN Number
Proper Shipping Name Environmentally hazardous substance, solid, n.o.s.
Packing Group (PG) III
DOT Pictograms
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Crystal Violet
[Ion association reagent for spectrophotometric analysis.]
Section XV. Other Regulatory Information and Pictograms
TSCA Chemical Inventory This compound is ON the EPA Toxic Substances Control Act (TSCA) inventory list.
(EPA)
WHMIS Classification On DSL.
(Canada)
EINECS Number (EEC) 208-953-6
EEC Risk Statements R23/24/25- Toxic by inhalation, in contact with skin and if swallowed.
R36/37/38- Irritating to eyes, respiratory system and skin.
R45- May cause cancer.
SECTION 16 - ADDITIONAL INFORMATION
N/A