Description:

Mouse monoclonal (Clone: 10P5) antibody

Background:

PHYB is a red/far-red photoreceptinvolved in the regulation of de-etiolation. It exists in two inter-convertible forms: Pr Pfr (active). It is involved in the light-promotion of seed germination in the shade avoidance response.

Synonyms:

PHYB, HY3, OOP1, OUT OF PHASE 1, PHYTOCHROME B

Immunogen:

Recombinant protein of PHYB derived from Arabidopsis thaliana AT2G18790.

Form:

Lyophilized

Quantity:

150 μg

Purification:

Protein A purified

Reconstitution:

Reconstitution with 150μl of 0.01M sterile PBS.
"Note: please spin tube briefly prito opening it to avoid any losses that might occur from lyophilized material adhering to the cap sides of the tube".

Stability &
Storage:

Use a manual defrost freezer avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Applications:

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

Expected Results:

125 kDa

Confirmed Reactivity:

Arabidopsis thaliana

Predicted Reactivity:

Fmore species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 35 μg total protein from phyB-9 mutant of Arabidopsis thaliana leaf.
Lane 2: 35 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 8% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane f1h.
Blocking: 5% skim milk at RT 4℃ f1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP).
Detection: using chemiluminescence substrate image were captured with CCD camera.