名称 Ponceau S 丽春红S
其它名称
英文名称：Ponceau S；C.I. 27195；3-Hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonic acid tetrasodium salt其他名称：猩红S；酸性红112；3-羟基-4-[2-磺酸基-4-(4-磺酸苯基偶氮)苯基偶氮]-2,7-萘二磺酸四钠盐
规格
PH1428-10 | 10g
产品简介
Ponceau S
CAS Number: 6226-79-5
Storage Temperature: Room Temperature
Product Description :
Appearance: Deep red crystalline powder
Molecular formula: C22H12N4Na4O13S4
Molecular weight: 760.6
Ponceau S is a red, water-soluble, anionic diazo dye that is made by coupling diazotized 4-amino-1,1’-azobenzene-3,4’-disulfonic acid (Acid Yellow 9) with 2-naphthol-3,6-disulfonic acid(R acid)converting the coupled product to the tetrasodium salt.
Ponceau S is a negative stain, which binds to the positivelyged amino groups of the protein is widely used in gel electrophoresis. It also binds non-covalently to non-polar regions in the protein.
Ponceau S may not be as sensitive of a protein stain as Coomassie Blue. It is superito Acid Fuchsin in the VanGieson stain.
Preparation Instructions :
It is soluble in water (1 mg/ml), yielding a clear solution.
Procedure:
A solution as low as 1% ponceau S can be made in 7% acetic acid (w/w) fstaining polyacrylamide gels. The gel can be left in this solution feight hours. However, prito placing the gel in this staining mixture, the proteins on the gels should be fixed with a fixative agent such as methanol:acetic acid TCA.
Destaining of the gel can occur in 7% (v/v) acetic acid:water mixture with many changes of the destaining solution.
Storage/Stability:
Recommended storage is at room temperature. The shelf-life should be at least two years.
Precautions Disclaimer :
FLaboratory Use Only. Not fdrug, household other uses.
*Important Note: This product as supplied is intended fresearch use only, not fuse in human, therapeutic diagnostic applications.
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