应用
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分离DNA和RNA期间使核苷酸酶蛋白失活。
去除内毒素对阳离子蛋白的约束,如溶菌酶和核糖核酸酶。
研究报告显示用于分离肝,酵母,绿豆线粒体
测定细胞膜上的酶
用于抗体标记,石蜡包埋组织切片中暴露抗原结合位点。
消化脑组织样本中的蛋白质,用于朊病毒的传染性海绵状脑病(TSE)的研究
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产品介绍
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蛋白酶K是一个稳定,高活性丝氨酸蛋白酶。从晶体和分子结构研究的证据表明这种酶属于枯草杆菌家族,带有活性催化位点催化三键(Asp39,His69 - Ser224)。它是在宽的的环境范围内保持稳定:pH值,缓冲盐,去垢剂(SDS),和温度。在0.1-0.5% SDS存在下,在分离DNA中,蛋白酶K保持活性,消化各种蛋白质和核酸酶。
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备注
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Unit Definition
One unit will hydrolyze urea-denatured hemoglobin to produce colequivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (colby Folin-Ciocalteu reagent).
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生化机理
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Proteinase K is a stable highly reactive serine protease. Evidence from crystal molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity will digest a variety of proteins nucleases in DNA preparations without compromising the integrity of the isolated DNA.
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别名
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蛋白酶K;Proteinase KEndopeptidase K Prok Protease K Tritirachium album proteinase K
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