at#:2112 Anti-DNA-RNA Hybrid [S9.6] Antibody 



	
	

		
		

			
			

				
				

					
					

						Synonyms:
					

				

				

					
					

						DNA-RNA hybrid; RNA/DNA hybrid
					

				

			

			

				
				

					
					

						Attribute:
					

				

				

					
					

						 MERGEFIELD Antibody_type Mouse Monoclonal  Antibody 
					

				

			

			

				
				

					
					

						Isotype:
					

				

				

					
					

						Mouse IgG2a
					

				

			

			

				
				

					
					

						Purity:
					

				

				

					
					

						Protein A/G  Purification
					

				

			

			

				
				

					
					

						Application:
					

				

				

					
					

						 MERGEFIELD Tested_applications ELISA, IP, IF, CHIP, Dot Blot
					

				

			

			

				
				

					
					

						Immunogen：
					

				

				

					
					

						ΦX174
  bacteriophage-derived synthetic DNA/RNA
					

				

			

			

				
				

					
					

						Buffer：
					

				

				

					
					

						PBS with
  0.1% sodium azide 50% glycerol,  pH
  7.2
					

				

			

			

				
				

					
					

						Storage:
					

				

				

					
					

						Store at -20℃. Do not
  aliquot
					

				

			

			

				
				

					
					

						Recommended Dilution:
					

				

				

					
					

						WB: 1:1000-5000
					

					

						IP: 1:50-500
					

					

						ChIP: 1:50-500
					

					

						IF: 1:100-500
					

				

			

			

				
				

					
					

						Background：
					

					

						The DNA-RNA
  hybrids are a natural occurrence within eukaryotic cells their level are
  high at sites of high transcriptional activity. They are non-canonical
  nucleic acid structures with transcriptional regulatory functions. Their
  presence is reported to predispose a locus toomosomal breakage. The S9.6 monoclonal
  antibody recognizes DNA-RNA hybrids (also known as R-loops) does not bind
  to single double stranded DNA. The antibody has high affinity fDNA-RNA
  hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of
  the antibody appears to be determined by a combination of sequence
  structural dependency since R-loop sequence affects binding affinity. 
					

				

			

		

	




	





	





	





	





	





	





	





	





	





	





	





	





	





	





	





	





	





	





	





	





	





	





	





	检测结果



	 



	





	
	

		Immunofluorescent
analysis of ( 4% PFA) fixed HeLa cells using DNA-RNA hybrid  MERGEFIELD
Antibody_type Mouse Monoclonal  Antibody [S9.6]   at dilution of 1:500
	

	

		Alexa Flu647- conjugated
AffiniPure Goat Anti-Mouse IgG(H+L)
	

	

		


	

	

		 
	

	

		


	

	

		
		

			Chromatin   immunoprecipitation
analysis of  HeLa cells genomic DNA(gDNA)
using DNA-RNA hybrid  MERGEFIELD Antibody_type Mouse Monoclonal 
		

		

			Antibody [S9.6]   at dilution of 1:200